Granulocyte Collection by Continuous-flow Filtration Leukapheresis

Granulocyte collection by continuous-flow filtration leukapheresis.

A safe and simple technique for the ing preformed leukocyte antibodies, as collection of large numbers of granucompared to the median increment of locytes from normal donors, based on 850/cu mm reported following transthe reversible adhesion of granulocytes fusion of leukocytes obtained by difto nylon wool filters, is described. The ferential centrifugation. The safety and procedure is well tol...

Granulocyte bactericidal capacity and chemotaxis as affected by continuous-flow centrifugation and filtration leukapheresis, steroid administration, and storage.

While granulocyte transfusions have been shown to be effective in the treatment of patients with infections, the effects of the leukapheresis collection procedure on certam granulocyte functions remain in question. We studied the bactericidal function of granulocytes collected by continuousflow centrifugation (CFC) and filtration Ieukapheresis (FL) with S. aureus, E. coil, and S. typhimurium. K...

Polymorphonuclear leukocytes prepared by continuous-flow filtration leukapheresis: viability and function.

Preparation of granulocytes for transfusion in high yield and relatively free of contamination by other cell types has been made possible by the technique of continuous-flow filtration Ieukaph.resis (CFFL). Since previous work suggested that granulocytes collected in this manner may have impaired viability and function, a detailed study of the bactericidal, metabolic, and chemotactic properties...

In vitro function and post-transfusion survival of granulocytes collected by continuous-flow centrifugation and by filtration leukapheresis.

The function of granulocytes collected by continuous-flow centrifugation (CFC) and by filtration leukapheresis (FL) was studied in vitro, and the post-transfusion recovery and intravascular survival of these cells was studied by autologous transfusion in normal donors. Granulocytes collected by both FL and CFC leukapheresis (CFCL) functioned normally in the quantitative nitroblue tetrazolium, o...

Filtration leukapheresis (FL) and granulocytes.